Comparison of functional productivity of GFPnt and its variants.

The data show the whole cell fluorescences of GFPnt (containing all the 5 internal Met), GFPnt-r2M (containing M153T and M233K mutations), GFPnt-r3M (containing M153T, M233K and M218A mutations), GFPnt-r4M (M78I, M88L, M153T and M233K mutations), GFPnt-r5M (M78I, M88L, M153T, M218A and M233K mutation) and GFPhs-r5M (GFPnt-r5M containing mutations for folding enhancement). The relative fluorescence (in arbitrary units) is the fluorescence of whole cells compared with the fluorescence of cells expressing GFPnt.