Comparison of circulating miRNAs predicted to target Bmal1 or other genes regulating Bmal1 expression with a highly abundant miRNA in serum, miR-16.

(A) Relative expression of miR-16, miR-142-3p, miR-152, miR-494, miR-135b, miR-135a and miR-34c in serum samples collected from mice at ZT 7 (n = 3). Bars denote real-time PCR determinations of serum miRNA levels (mean ± SEM) and the values are plotted using a logarithmic scale in comparison with the average for miR-34c expression. (B) Quantification of miR-16 and miR-152 expression in serum collected from mice at ZT 7 (n = 3). Symbols denote determinations (in duplicate) of the number of copies/µl serum in each sample that were extrapolated by comparing the Ct values for experimental samples with standard curves consisting of a dilution series of known quantities of synthetic miR-16 and miR-152 analyzed on the same plate.