Comparison of T. brucei and T. congolense in vitro growth.
A, T. brucei 427 BSF have been inoculated at 5.104 cell/ml in TcBSF-1 (Table 1) medium at 37°C in a humidified atmosphere containing 5% (vol/vol) CO2. B, T. congolense STIB910 BSF have been inoculated at 5.104 in presence of BAE (black curves) or at 2.105 cell/ml in absence of BAE (grey curves) in TcBSF-2 (Table 1) medium at 34°C in a humidified atmosphere containing 5% (vol/vol) CO2. Parasites were cultured in their specific medium for five days (——⧫) or in medium supplemented with red blood cell lysate (resuspension of red blood cells vol/vol in cold water and 100 fold diluted in medium) (——•), or with hemoglobin (100 µg/ml) (—), or in absence of reducing agents (Bathocuproin, 2-mercaptoethanol and cystein were removed) (——▪). Cells were counted every 24 h.