Comparison between pentaplex PCR and NCI panel of markers to determine MMR-deficiency in colorectal cancers.
A) The figure shows the performance comparison between the NCI panel markers and the QMVR optimized pentaplex PCR. Black squares indicate a tumor positive for allelic variation (or unstable) and white squares indicate a tumor negative for any allele variations (or stable). The dinucleotide repeat markers (D2S123, D5S346 and D17S250) are less robust than the mononucleotide repeats for detecting MSI. B) The figure illustrates the frequency of tumors with number of markers displaying allelic variations in MMR-deficient and proficient CRCs. As indicated, pentaplex PCR shows a higher sensitivity and specificity compared to NCI panel of markers, and the distribution of altered markers is unambiguously bimodal.