Collagen 1 and 3 mRNA expression determined by RT-PCR.
Stacked bar charts of the relative mRNA amounts, collagen 1 (white bar) and 3 (black bar), recorded for Bard meshTM (A), PhysiomeshTM (B), the new composite mesh (C) and VentralightTM (D) at the different study times (left panel). Results are the mean ± SEM of three experiments. Gene expression was normalized to that of the GAPDH gene. Agarose gels obtained (right panel). Mw: molecular weight markers; 1/2: 14 days postimplant; 3/4: 90 days postimplant; N: negative. Col 1: *, p<0.05 and **, p<0.01 vs. BardTM at 90 days; #, p<0.01 vs. new composite mesh at 14 days. Col 3: |, p<0.05 vs. BardTM at 90 days; †, p<0.05 and ††, p<0.01 vs. PhysiomeshTM at 14 days; ‡, p<0.05 vs. new composite mesh at 14 days.