Co-immunoprecipitation of SOLO and SMC1 from ovarian extracts.

2013-07-18T03:49:41Z (GMT) by Rihui Yan Bruce D. McKee

Ovary lysates were prepared from y w and FH::SOLO ({UASp-FH::SOLO}/CyO; {nos-GAL4::VP16}/TM2) ovaries and used for Western blot and immunoprecipitation experiments. (A) Western blot of protein extracts from FH::SOLO and y w ovaries using anti-FLAG antibody. Absence of signal in the y w lane shows that the anti-FLAG antibody is specific. (B) Immunoprecipitation with anti-SMC1 antibody from protein extracts from FH::SOLO and y w ovaries, analyzed with anti-FLAG antibody. (C) Immunoprecipitation with anti-SMC1 antibody or rabbit serum (mimic control) from FH::SOLO ovary extracts, analyzed with anti-FLAG antibody. (D) Immunoprecipitation with anti-FLAG antibody or mouse serum (mimic control) from FH::SOLO ovary extracts, analyzed with anti-SMC1 antibody.