Citral inhibition of I<sub>TRPV1</sub> reveals the expression of TRPV2 and TRPV3 in acutely isolated dorsal root ganglion neurons.
2008-05-07T00:50:24Z (GMT) by
<p>(A) Two patterns of response to repetitive 2-aminoethyldiphenyl borate application were noted within the dorsal root ganglion population following citral block of capsaicin-activated currents; dorsal root ganglion currents decreased in six neurons (left), and increased in the remaining neurons (right; n = 6; +100 mV). Holding potential = 0 mV. (B) Representative dorsal root ganglion currents from a neuron not potentiated by repeated 2-aminoethyldiphenyl borate application are shown. (C) Representative dorsal root ganglion currents from a neuron potentiated (2.3 fold; +100 mV) by repeated 2-aminoethyldiphenyl borate application are shown. (D) TRPV2 immunoreactivity in dorsal root ganglion neurons stained with 0.3 µg/ml anti-TRPV2 antibody+Alexa 488 secondary is shown (left; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0002082#pone.0002082.s005" target="_blank"><i>Figure S5</i></a> for controls). Differential interference contrast image (right).</p>