Citral inhibition of ITRPV1 reveals the expression of TRPV2 and TRPV3 in acutely isolated dorsal root ganglion neurons.
(A) Two patterns of response to repetitive 2-aminoethyldiphenyl borate application were noted within the dorsal root ganglion population following citral block of capsaicin-activated currents; dorsal root ganglion currents decreased in six neurons (left), and increased in the remaining neurons (right; n = 6; +100 mV). Holding potential = 0 mV. (B) Representative dorsal root ganglion currents from a neuron not potentiated by repeated 2-aminoethyldiphenyl borate application are shown. (C) Representative dorsal root ganglion currents from a neuron potentiated (2.3 fold; +100 mV) by repeated 2-aminoethyldiphenyl borate application are shown. (D) TRPV2 immunoreactivity in dorsal root ganglion neurons stained with 0.3 µg/ml anti-TRPV2 antibody+Alexa 488 secondary is shown (left; see Figure S5 for controls). Differential interference contrast image (right).
