Fig_1.tif (627.56 kB)

Cigarette smoke condensate (CSC) activates the HPV-16 p97 promoter in a dose-dependent manner in lung and cervical cancer cells.

Download (0 kB)
posted on 01.04.2015 by Nelson Peña, Diego Carrillo, Juan P. Muñoz, Jonás Chnaiderman, Ulises Urzúa, Oscar León, Maria L. Tornesello, Alejandro H. Corvalán, Ricardo Soto-Rifo, Francisco Aguayo

(A) A-549 (B) H-2170 (C) HeLa and (D) SiHa cells were exposed to CSC (0.1–50 μg/mL) for 16 hours after transfection with the pmiR-GLO construct containing the complete HPV-16 LCR/p97 region. (E) A-549 cells were transfected with the pmiR-GLO containing only the HPV-16 p97 promoter and the luciferase activity was compared with those cells transfected with the full HPV-16 LCR/p97 region. (F) SiHa cells were exposed to 10 μg/mL CSC and the levels of E7 transcripts were measured using RT-qPCR. The graphs are representative of three independent experiments. (* = p<0.05; ** = p<0.01; *** = p<0.001, Luc2/Reni percentage ± SEM).