Figure_3.tif (201.21 kB)
Characterization of C3201-HSA fusions.
figure
posted on 2013-04-22, 02:11 authored by Richard Smith, Amy Duguay, Alice Bakker, Peng Li, Jennifer Weiszmann, Melissa R. Thomas, Benjamin M. Alba, Xinle Wu, Jamila Gupte, Li Yang, Jennitte Stevens, Agnes Hamburger, Stephen Smith, Jiyun Chen, Renee Komorowski, Kevin W. Moore, Murielle M. Véniant, Yang Li(A) Fusions of C3201 and HSA induce luciferase activity on an FGF21-responsive engineered reporter cell line following four hour stimulation of serum-starved cells. Potency is influenced by the orientation of the fusion. Luciferase activity is measured in relative luminescence units (RLU). Data are represented as mean +/− SEM (n = 2). (B) Fusions of C3201 and HSA induce ERK phosphorylation in cultured primary human adipocytes. Potency is influenced by the orientation of the fusion. Data are expressed as % ERK protein that is phosphorylated, as measured by MSD.
