Changes in PLDδ abundance in detached Arabidopsis leaves floated on water, ABA, or ethylene.

Total protein (40 µg) was extracted from WS leaves treated with either water, 50 µM ABA, or 50 µM ethephon (ETH) for 0, 3, and 5 days and separated by SDS-PAGE. PLDδ was detected by western blotting using a PLDδ-specific antibody. Protein from PLDδ-KO leaves that underwent no additional treatment is shown as a negative control.