Central RPE cells do not inhibit peripheral RPE cells from proliferating via diffusible signals.

RPE cultures were setup from peripheral and central RPE and were allowed to proliferate at low density for one week, before peripheral RPE cultures were introduced to central RPE medium for two days before a 4-hour BrdU pulse. (A) The graph indicates the number of BrdU⁺ cells per region per total cell number. Periphery (circle points), central (square points) and control periphery (triangle points). Cell number per culture; 5,000 cells/well. Mann-Whitney Test; *p = 0.03. **p = 0.02. Peripheral RPE versus control RPE periphery was not found to be statistically significant (p = 0.68). Error bars = SD. (B) RPE cultures were setup from peripheral and central RPE and allowed to proliferate at high density for one week, before peripheral RPE cultures were introduced to central RPE medium for two days before a 4-hour BrdU pulse. The graph indicates the number of BrdU⁺ cells per region. Periphery (circle points), central (square points) and control periphery (triangle points). Cell number per culture; 10,000 cells/well. Error bars = SD.