Cell spreading promotes TGFβ1-induced expression of actin binding proteins and myofibroblast markers.
(A) Immunofluorescence staining of αSMA, caldesmon, and tropomyosin for TGFβ1-treated cells cultured on 400 μm2 and 2500 μm2 squares. Stamped protein islands are outlined by a white dotted line. (B) Frequency of squares with αSMA expression after treatment with and without TGFβ1. Relative levels of (C) caldesmon and (D) tropomyosin with and without TGFβ1. Relative levels are computed in comparison to 400 μm2 control samples. *p < 0.05; ***p < 0.001. Scale bars, 20 μm.