Cell shape regulates TGFβ1-induced expression of αSMA.
(A) Immunofluorescence staining of αSMA for TGFβ1-treated NMuMG cells on 1600 μm2 islands with shape factors 1 and 8. (B) Quantification of the percentage of NMuMG cells expressing αSMA on islands with shape factors 1 and 8 after a 48 h treatment with or without TGFβ1. *p < 0.05, **p < 0.01. Scale bars, 20 μm.