Figure_4.tif (347.79 kB)
Caveolin-1 and cholesterol dependent viral entry.
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posted on 2013-10-11, 03:02 authored by Mohammad A. Yousuf, Xiaohong Zhou, Santanu Mukherjee, Ashish V. Chintakuntlawar, Jeong Yoon Lee, Mirja Ramke, James Chodosh, Jaya RajaiyasiRNA and scrambled (sc)RNA were generated and transfected into human corneal cells. A. Real-time RT-PCR for caveolin-1 mRNA confirmed successful reduction of caveolin-1 message (~95%) as compared to scRNA transfected cells (*p=.0002). B. Western blot showed reduced expression of caveolin-1 in caveolin-1 specific siRNA treated cells (right lane) as compared to untransfected and uninfected cells (left lane) or scRNA transfected, virus infected cells (middle lane) C. IL-8 mRNA expression after HAdV-D37 infection was reduced to almost 50% by caveolin-1 siRNA transfection, compared to scRNA (*p=.0001).