Call rate per pneumococcal genomic fragment.
The upper panel shows the resequencing profile of the eleven genomic fragments tiled on the chip and analyzed from 72 strains in duplicates. The variability in call rate increases as the query sequence diverges from the reference on the chip. The vertical bars represent the standard deviation in the results. The lower panel shows the complementation of sequence information using complementary ABI Sanger sequencing method for the genomic fragments. Cumulative data obtained was ≥95% sequence information per fragment (Table S2). Light blue bars: resequencing array platform; dark red bars: cumulative data from resequencing array and Sanger sequencing platforms.