Calibration curves of fluorescence intensity and concentrations of four recombinant viral antigens.

A. The fluorescence signals scanned at different concentrations of antigen HSV1-gG from 1.56, 3.125, 6.25, 12.5, 25 µg/ml to 50 µg/ml; B. The fluorescence signals scanned at different concentrations of antigen HSV2-gG from 1.56, 3.125, 6.25, 12.5, 25 µg/ml to 50 µg/ml; C. The fluorescence signals scanned at different concentrations of antigen CMV-pp150 from 1.56, 3.125, 6.25, 12.5, 25 µg/ml to 50 µg/ml; D. The fluorescence signals scanned at different concentrations of antigen RV-EP from 0.31, 0.625, 1.25 2.5, 5, 10 µg/ml; the equation and the coefficient of determination (R²) are indicated for each curve.