CXC chemokine receptor 4 (CXCR4) and CXC chemokine ligand 12 (CXCL12) expression.
(A) Representative immunofluorescence stainings for CXCR4 and von Willebrand Factor (vWF) showing that while CXCR4 was found on vWF+ endothelial cells (arrow), perivascular cells and cells in the alveolar walls in the lungs of control animals, there was an increase in CXCR4 expression in luminal, vessel wall and perivascular cells with multiple CXCR4+ vWF+ cells (arrows) in the lumen of the pulmonary artery in the lungs of SuHx animals. Please note that the vessel shown in the image of SuHx day 6 was sectioned transversally, whereas the vessels in the images of SuHx day 21 and 42 were sectioned in a more longitudinal manner. Nuclear staining with 4',6-diamidino-2-phenylindole (DAPI). Magnification: 630×. Scale bar: 20 µm. (B) Representative Western blot analysis indicates that the protein expression of the CXCR4 ligand CXCL12 was increased in SuHx lung tissue protein lysate as compared to naïve control animals. β-actin was used as loading control. (C) Densitometry of the Western blot in (B). Densitometric values were normalized vs. β-actin and expressed as n-fold of naïve controls. n = 3 animals per group. * P<0.05.