COA, but not N2A, results in S-phase arrest of cell cycle.
Cells were incubated under aerobic and anaerobic conditions, N2A or COA, for 4 days. Insert: representative FACS analysis of cell cycle for K562 cells incubated under different atmospheres. Arrows indicate center of S-phase. Main Figure: phase distribution of viable cells. Mean ± SD from 3 independent experiments is shown. Empty bars - G0/G1 phase, black bars - S phase and grey bars - G2/M phase.