CCoV-II infection modulates TRAIL protein expression.
(A) Cell lysates were collected at the indicated times p.i., and equal amounts of proteins from each sample were subjected to Western blot analysis and probed for TRAIL antibody which recognized the 28–30 KDa bands for TRAIL-R1 and TRAIL-R2, respectively. β-actin was used as a loading control. The molecular weight (KDa) of protein size standards is shown on the left hand side. Blot is representative of three separate experiments. (B) Densitometric analysis of blots relative to TRAIL 30 and TRAIL 28. The bars represent the mean ± SEM of the results from three separate experiments. Significant differences between CCoV-II-infected cells and control cells are indicated by probability P. **P <0.01, and ***P <0.001.