Binding of the dopamine D2 receptor C-terminus to NCS-1 monitored using tryptophan fluorescence.

(A) The spectra represent intrinsic tryptophan fluorescence of NCS-1 before and after addition 1 µM Ca²⁺ and after subsequent addition of the D2SR peptide. (B) Extent of the changes in tryptophan fluorescence following sequential additions of D2SR peptide to the final concentrations indicated. The data are shown as mean±SEM (n = 5) and were fitted using a logistic fit by non-linear curve fitting.