Binding of SsbB protein to dT_n oligomers in the presence of Mg²⁺.

The reaction solutions contained 25 mM Tris acetate (pH 7.5), 10 mM magnesium acetate, 5 µM dT_n (nucleotide concentration), and the indicated concentrations of SsbB protein (tetramer concentrations). The reactions were analyzed by polyacrylamide gel electrophoresis using a gel running buffer consisting of Tris acetate (pH 7.5) and 10 mM magnesium acetate. The bands corresponding to the unbound dT_n oligomers, and the B₁ and B₂ complexes, were visualized by phosphorimaging.