Binding of F4ab fimbriae to reference glycosphingolipids.

<p>Chemical detection by anisaldehyde (A), and autoradiograms obtained by binding of <sup>125</sup>I-labeled F4ab fimbriae (B). The lanes were: Lane 1, sulfatide (SO<sub>3</sub>-3Galß1Cer) with d18:1-24:1 ceramide, 4 µg; Lane 2, sulfatide with d18:1-h16:0 ceramide, 4 µg; Lane 3, sulfatide with t18:0-h24:0 ceramide, 4 µg; Lane 4, sulf-lactosylceramide (SO<sub>3</sub>-3Galß4Glcß1Cer) with t18:0-h16:0 ceramide, 4 µg; Lane 5, reference sulf-gangliotetraosylceramide (SO<sub>3</sub>-3Galß3GalNAcß4Galß4Glcß1Cer), 4 µg. The glycosphingolipids visualized with anisaldehyde in (A) are marked with Roman numbers, and the corresponding glycosphingolipid structures are given below the chromatograms.</p>

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