BCL6 downregulation in CD11cint I-Ahi cells is correlated to reduced proliferation.
Splenocytes or LN cells obtained before and after Alum/CFA inoculation were stained with BCL6, Ki-67 and cDC markers. (A) Representative FACS plots (n = 4) showing Ki-67 and BCL6 expression on gated either CD11chi I-Aint or CD11cint I-Ahi splenic cDCs at day 0, 2 and 7 after Alum i.p. (B) The average MFI of Ki-67±SEM (n = 4) in either CD11chi I-Aint (solid black bar) or CD11cint I-Ahi (open bar) splenic cDCs. (C) and (D) Representative flow cytometry data (n = 4) showing Ki-67 and BCL6 expression in CD11chi I-Aint or CD11cint I-Ahi cells of mesenteric LNs under steady state (C) or of draining popliteal LNs two days post CFA injection (D). *p<0.05, ***p<0.001 (Two way ANOVA) was compared either as indicated or with its counterpart.