Autophagy induced by TLR2 or TLR7/8 ligand in Rab39a-KD macrophages.

(A) Immunostaining analysis of Rab39a-KD macrophages stimulated by TLR2 or TLR7/8 ligand. Raw264.7 macrophages transfected with control or Rab39a siRNA were treated with TLR2 ligand Pam3CSK4 at 10 ng/ml or TLR7 ligand R848 at 1 μg/ml for 24 h and immunostained with anti-LC3 antibody. (B) Proportion of Raw264.7 macrophages with LC3-dots induced by Pam3CSK4 or R848 for 24 h. (C) Immunoblot analysis of LC3 processing in macrophages treated with Pam3CSK4 or R848. Raw264.7 macrophages transfected with control or Rab39a siRNA were treated with Pam3CSK4 or R848 for 24 h and subjected to immunoblot analysis using indicated antibodies. Data represent the mean and SD of three or four independent experiments. *p < 0.05 (unpaired Student’s t-test).