Autophagic inhibition accumulates GFPu in a p62 dependent manner.
Cultured NRVMs were infected with adenoviruses to express GFPu and RFP (Ad-GFPu/RFP) 24 hours before transfection with siRNA for Atg7 (siAtg7), Rab7 (siRab7) and/or for p62 (sip62). A luciferase-specific siRNA (siLuc) was used as control for siRNA infection and off-target effects. The cells were harvested at 48 h later for extracting total proteins. A, Shown are representative images out of 3 repeats. B, pooled densitometry data. *p<0.05 vs. CTL.