Asymmetric expression and function of hlh-14 in the C-lineage.

A: Ventral view of the 3D-positions of certain cells of the C-lineage of a representative wild-type embryo at the AB256-cell stage (shortly after the division of the ASE grandmothers), as assessed by 4D-lineage analysis. The left-sided asymmetric neuroblast Caapa and the neuron PVR express hlh-14 (purple asterisks). Cells of the gut and the ASE lineages are shown for positional information. Cell coloring, except C-lineage, after [10]. B: Ventral view in both DIC and fluorescence of a representative lineaged hlh-14(tm295); Ex[hlh-14FOS::yfp]; ntIs1 embryo at the AB256-cell stage. C: Panel shows the expression (purple lines) of hlh-14 and the division pattern of part of the C-lineage as determined by 4D-lineage analysis of wild-type (containing the ntIs1 transgene, hlh-14(gm34); otIs114, hlh-14(tm295); ntIs1 and hlh-14(tm295); otEx4507, Ex[hlh-14FOS::yfp]; ntIs1 embryos. hlh-14 expression was assessed in both gmIs20 and hlh-14(tm295); Ex[hlh-14FOS::yfp] animals. The Caapa blastomere divides early in hlh-14 mutants, at a time when closely related cells produce hypodermal cells. This defect is rescued by the hlh-14FOS::yfp transgene. The extra hyp11 cell that can be observed expressing dpy-7 (Figure S3), is indicated. The number of lineages that divided as indicated and the total number of individual left or right lineages followed to terminal division are shown at the bottom of each lineage diagram. Red cell names indicate neurons/glia and yellow cell names indicate hypodermis.