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Association of TopBP1 and Cdc45 with DNA.

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posted on 2011-07-29, 00:43 authored by Seçil Yilmaz, Aziz Sancar, Michael G. Kemp

The association of each of the indicated purified proteins with beads alone (No DNA) or to single-stranded (ss), double-stranded (ds), and branched, replication fork-like (fork) DNA bound to magnetic beads was analyzed by SDS-PAGE and immunoblotting. Each of the following proteins was analyzed for binding to each DNA structure: (A) TopBP1 and (B) Cdc45. Reactions were carried out as described in the Methods section.

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