Assessment of astrocyte viability in response to 12 hours of oxygen- glucose deprivation.

(1A) Astrocyte viability was assessed using an MTT assay and did not show a difference in absorbance between the astrocytes exposed to OGD-alone compared to the astrocytes that were exposed to OGD with relaxin-2 (10, 50 ng/mL), relaxin-3 (10, 50 ng/mL) or R3/I5 (10, 50 ng/mL). (1B) Astrocytes that were exposed to relaxin-2, relaxin-3, and R3/I5 during the 24 hour OGD protocol all demonstrated an increased viability when compared to untreated astrocytes that were exposed to OGD for 24 hours. (1C) Finally, astrocytes that were exposed to relaxin-2, relaxin-3, and R3/I5 during the 48 hour OGD protocol did not exhibit different viability or mitochondrial uptake of MTT compared to untreated astrocytes that were exposed to OGD. The * indicates significantly different from OGD astrocytes.