Assays for MAP kinase phosphorylation.
Western blots were conducted with proteins isolated from vegetative hyphae of the wild-type (70-15) and the Momsb2 (M6), Mosho1 (S72), and Mosho1 Momsb2 (MS88) mutant strains. A. The anti-MAPK and anti-TpEY antibodies detected the expression and phosphorylation levels of Pmk1 (42-kD) and Mps1 (46-kD), respectively. B. The 41-kD Osm1 band was detected with both an anti-P38 MAPK antibody and the anti-TpGY antibody.