Arginine residues in and near the D-box are required for cyclin B1 localization to mitotic chromosomes.

A. Representative images of mitotic BS-C-1 cells expressing cyclin B1 containing mutations in and near the D-box. White arrows indicate the position of the metaphase plate as seen in the DIC image. Scale bar = 10 µm. B. CER box-and-whisker plots for the constructs shown in part A. The red dotted line indicates the threshold of 1.6. N  = 10–14, * indicates p<0.05, ** indicates p<0.01 and *** indicated p<0.001 compared to WT^1–433. Further details including mean CER, standard deviations, and Wilcoxon exact test p-values can be found in Table S2. C. Representative images of mitotic BS-C-1 cells expressing cyclin B1 fragments bearing R42A or Δ3–8/R42A double mutations. White arrows indicate the position of the metaphase plate as seen in the DIC image. Scale bar = 10 µm. D. CER box-and-whisker plots for the constructs shown in part C. The red dotted line indicates the threshold of 1.6. N = 9–13, * indicates p<0.05, ** indicated p<0.01 and ***indicates p<0.001 compared to WT counterpart. Further details including mean CER, standard deviations, and Wilcoxon exact test p-values can be found in Table S2.