Aquaporin-5 and 8-hydroxy-2′ deoxyguanosine (8-OHdG) expression in the lungs after hemorrhagic shock and resuscitation (HSR).
Sections of lungs obtained 3 h after resuscitation were subjected to fluorescent immunohistochemical analysis for 8-OHdG (green) and aquaporin-5 (red). Above row: 8-OHdG-positive cells in the vehicle/sham (A), BV/sham (B), vehicle/HSR (C) and BV/HSR groups (D). Middle row: aquaporin-5-positive cells in the vehicle/sham (E), BV/HSR (F), vehicle/HSR (G), and BV/HSR groups (H). Bottom row: merged images of 8-OHdG and aquaporin-5 in the vehicle/sham (I), BV/sham (J), vehicle/HSR (K), and BV/HSR groups (L). Vehicle/sham, vehicle-administered animals subjected to sham surgery; BV/Sham, BV-administered animals subjected to sham surgery; vehicle/HSR, vehicle-administered animals subjected to HSR; BV/HSR, BV-administered animals subjected to HSR. Scale bar = 50 µm. All images shown were visualized with a 20×0.5 objective lens.