Appearance of cultured nasal epithelial cells at passage 3.

(A) Phase contrast light micrographs showing typical “cobblestone” morphology of epithelial cells (size marker  = 400 µm). (B) Scanning and (C) transmision electron micrographs (size marker  =  20 µm and 2 µm respectively). Microvilli (MV) and tonofilaments (TF) indicated within cell ultrastucture. (D) and (E) positive immunostaining for cytokertain 19 and cytokeratin 13 respectively (green) and DAPI (blue) (size marker  =  50 µm).

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