Figure_3.tif (1.99 MB)
ApoEdp inhibited capillary tubule formation (in vitro angiogenesis).
figure
posted on 2013-02-20, 21:24 authored by Partha S. Bhattacharjee, Tashfin S. Huq, Tarun K. Mandal, Richard A. Graves, Syed Muniruzzaman, Christian Clement, Harris E. McFerrin, James M. HillAbout 4×104 (per well) HUVECs in medium containing 50 ng/mL of VEGF were plated in 24-well plates previously coated with growth factor-reduced matrigel, and incubated for 12–16 hr at 37°C in the absence or presence of apoEdp. Tubular structures were quantitated by manual counting under low power fields. Representative photomicrographs of tubule formation in the (A) VEGF control and (B) apoEdp-treated wells are shown. (C) “Percentage (%) of inhibition” is the mean number (± SEM) of tubules expressed as a proportion of that in the VEGF control group. ** p<0.01 and *** p<0.001.
