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Antigen (PPD and Flu)-specific CD4+ T-cell responses in peripheral blood during ART.

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posted on 2014-06-24, 14:56 authored by Enoch Sepako, Sarah J. Glennie, Kondwani C. Jambo, David Mzinza, Oluwadamilola H. Iwajomo, Dominic Banda, Joep J. van Oosterhout, Neil A. Williams, Stephen B. Gordon, Robert S. Heyderman

Patients were analysed prior to initiation of ART and followed-up at 3, 6 and 12 mths ART for CD4⁺ T-cell responses. Responses from HIV-infected persons at 12 months ART were compared with HIV-negative persons recruited from the same community (A) ex vivo IFN-γ ELISpot responses to M.tuberculosis PPD (0mth, n = 36; 3mths, n = 36; 6mths, n = 26; 12mths, n = 10; HIV-, 24) (B) ex vivo IFN-γ ELISpot responses to influenza antigens (0mth, n = 36; 3mths, n = 36; 6mths, n = 26; 12mths, n = 10; HIV-, 24) (C) proliferative responses (8 day CFSE dilution assay) to M.tuberculosis PPD (0mth, n = 12; 6mths, n = 12; 12mths, n = 12; HIV-, 28) (D) proliferative responses to influenza antigens (0mth, n = 14; 6mths, n = 12; 12mths, n = 13; HIV-, 28) (E) CD154 expression on activated CD4⁺ T cells in response to M.tuberculosis PPD (0mth, n = 33; 3mths, n = 30; 6mths, n = 30; 12mths, n = 16; HIV-, 29) (F) CD154 expression on activated CD4⁺ T cells in response to influenza antigens (0mth, n = 30; 3mths, n = 28; 6mths, n = 30; 12mths, n = 16; HIV-, 29). Black horizontal bars represent median and IQR after background responses were substrated from all antigen-specific CD4⁺ T-cell responses. Wilcoxon matched pairs were used to compare T cell characteristics of HIV-infected persons on ART over time, and the Mann Whitney U test in the HIV-infected and HIV-uninfected comparisons. Representative flow cytometric data demonstrating CD4⁺ T cell proliferative responses and CD154 expression and gating strategy are shown in Figure S1.