Analysis of ribosome occupancy and ribosome density in HEK293T cells.
(A) The number of genes as a function of ribosome occupancy. The average ribosome occupancy is 85%. (B) The number of genes as a function of ribosome density. On average, there is one ribosome per 189 nucleotides. (C) Ribosome density as a function of a gene's coding sequence length. Each gene is indicated by a blue circle. The red line indicates the moving average density value (window of 50). The inset shows only genes with coding sequences that are shorter than 1,000 nucleotides.