Analysis of rRNA levels in strains expressing Nop8p truncated mutants by northern hybridization.

(A) Total RNA was extracted from cells growing in galactose (t0) or in glucose-containing medium for 10 or 18 hours and subjected to northern hybridization with probes specific for mature rRNAs. (B) Relative rRNA levels were obtained after quantitation of the bands in a Phosphorimager. Values correspond to rRNA levels corrected for the scR1 RNA; t/t0.