Analyses of enzymatic activity involved in catechins galloylated and hydrolysis biosynthesis in different organs and leaves at different developmental stages.

The enzyme activity unit was defined as the amount of product formed by one milligram of enzyme catalyzed in one minute (µg min−1 mg−1 protein); The UGGT activity was assayed with the substrates gallic acid. The ECGT activity was assayed with the substrates EGC and β-glucogallin. The GCH activity was assayed with the substrate EGCG.