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An overview of experimental flow identifying initial optimisation of appropriate EMS treatment (A) and development of the control and mutant populations for reverse genetic screening for SNP discovery and validation.
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posted on 2013-12-18, 03:21 authored by Frances M. Shapter, Michael Cross, Gary Ablett, Sylvia Malory, Ian H. Chivers, Graham J. King, Robert J. HenryNote, mutant generational nomenclature is as per McCallum et al 2000, where d denotes dosage trial, M indicates EMS treated seed and S indicates the selfed control line.
