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Amphibians from Switzerland sampled for skin peptide effectiveness and mucosome function against Bd, and Bd infection prevalence at different life-history stages.

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posted on 2014-04-30, 03:10 authored by Douglas C. Woodhams, Hannelore Brandt, Simone Baumgartner, Jos Kielgast, Eliane Küpfer, Ursina Tobler, Leyla R. Davis, Benedikt R. Schmidt, Christian Bel, Sandro Hodel, Rob Knight, Valerie McKenzie

Skin peptide effectiveness is the percent inhibition of Bd zoospore growth caused by 50 µg/ml peptide multiplied by the quantity of peptides (mg) per g amphibian according to Woodhams et al. [11]. The mucosome function against Bd (Swiss isolate TG 739) is a measure of zoospore viability quantified by the ratio of green:red fluorescence as described above. Infection prevalence is the mean from all amphibians in each group from multiple sites and seasons.

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Larval and post-metamorphic skin peptide samples extracted by different methods.

*Peptide effectiveness  = % inhibition of Bd growth at 50 µg/ml * mg peptides/g frog mass.

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Includes samples from chytridiomycosis outbreak sites in Spain (S. Walker, unpubl.), not included in logistic regression.

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