Amino acid substitutes in YebN cytoplasmic regions alter Xoo Mn²⁺ tolerance.

The strains that yebN mutant (ΔyebN) carries extrachromosomal yebN wild type sequence (WT) or some point mutations in YebN cytoplasmic regions (G25A, A26N, A27N, A27ND35A and G167A) cloned into the pHM1 vector were analyzed as described in Figure 2A. The strain that ΔyebN contains the pHM1 vector was the negative control (CK).