Amino acid sequence alignment of potential catalytic domains of CDA with orthologs from other insect species.

Partial amino acid sequences (annotations are shown according to NCBI reference sequences) from Anopheles gambiae (XP_316929), Culex quinquefasciatus (XP_001848571 and XP_001868428), Drosophila melanogaster (NP_001097045), and Tribolium castaneum (NP_001103739) were aligned with IsCDA using the GENETYX software. The chitin-binding and catalytic domains are indicated with dark lines, while CDA motifs are denoted by gray boxes.