Aldicarb hypersensitivity in worms during cholesterol starvation or <i>lrp-1</i> RNAi.

2012-02-21T02:27:41Z (GMT) by Mary Wiese Adam Antebi Hui Zheng
<p>A) The second generation of worms on a cholesterol starvation diet arrest at the L1/L2 stage and are aldicarb hypersensitive. B) Worms grown on a cholesterol-free diet for one generation were tested on aldicarb as young adults. Excess cholesterol (50 µg/ml) had no effect on aldicarb sensitivity, however cholesterol starved animals are aldicarb hypersensitive. C) Worms grown on <i>lrp-1</i> RNAi also experience aldicarb hypersensitivity. Control strains for these experiments are as follows: <i>unc-32(e189)</i> - mutant for a vacuolar H<sup>+</sup>-ATPase, aldicarb resistant; <i>dgk-1(nu62)</i> - diacylglycerol kinase mutant, aldicarb hypersensitive; <i>unc-29(e1072)</i> - non-alpha subunit of the nicotinic acetylcholine receptor, aldicarb resistant. Each experiment was performed at least three times (n = 50 per strain). Error bars represent the s.e.m.</p>