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Adaptive immunity features from cynomolgus macaques naturally infected with T. cruzi (CH) and non-infected controls (NI).
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posted on 2016-01-28, 12:41 authored by Renato Sathler-Avelar, Danielle Marquete Vitelli-Avelar, Armanda Moreira Mattoso-Barbosa, Marcelo Perdigão-de-Oliveira, Ronaldo Peres Costa, Silvana Maria Elói-Santos, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Andréa Teixeira-Carvalho, Olindo Assis Martins-Filho, Edward J. Dick Jr, Gene B. Hubbard, Jane F. VandeBerg, John L. VandeBerg(A) The frequencies of CD3+ lymphocytes and T-cell subsets (CD4+ and CD8+), the expression of adhesion molecule (CD54) and activation status (CD69 and HLADR) were performed by multicolor flow cytometry. (B) The expression of cytotoxicity markers (Granzyme A, Granzyme B and Perforin) of CD8+ T-cells was investigated by intracellular staining flow cytometry. (C) Analysis of B-cells, the activation status (CD69), and the expression of the regulatory FcγR (CD32) were evaluated by three-color flow cytometry. The results are expressed as mean percentage with standard error. Significant differences at p<0.05 are identified by (*).
