Activation upregulates 4-1BB expression in PDCA-1+ cells.

A Total BM-derived cells were stimulated with the indicated soluble agonists for 3d, stained with fluorochrome- tagged Abs to 4-1BB, PDCA-1, and IgD, then analysed by flow cytometry. The percentages of 4-1BB+ cells in gated PDCA-1+IgD+ and PDCA-1+IgD fractions are presented as bar graphs (mean ± SD). The results of three pooled independent experiments are shown (n = 3). B Purified splenic PDCA-1+ B cells from naïve B6 mice were stimulated with anti-4-1BB (5 µg/ml) for 3d, stained as indicated, and flow cytometry was performed. The frequencies of cells expressing the indicated activation markers were evaluated and are presented as bar graphs (mean ± SD). The results of three pooled independent experiments are shown (n = 3). * p<0.05.

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