Activation of p53 and Trp53-dependent proliferation arrest in Zfp148gt/gt MEFs.

(A) Western blots of MEF lysates showing expression of phospho-p53Ser18 in wt and Zfp148gt/gt MEFs cultured at 21 or 3% oxygen, respectively. Trp53−/− cells were used as a negative control and actin was used as a loading control. Asterisk indicates unspecific band. (B) Real-time RT-PCR of p21 in wt and Zfp148gt/gt MEFs cultured at 21 or 3% oxygen, respectively, and on Trp53+/+, Trp53+/− and Trp53−/− genetic backgrounds (n = 4). (C, D) CPD of Zfp148gt/gt and wt MEFs on Trp53+/+ or Trp53−/− (C) and Trp53+/+ or Trp53+/− (D) genetic backgrounds (n = 3). (E, F) CPD of Zfp148gt/gt and wt MEFs supplemented with n-acetyl-L-cysteine (NAC) (E) in the culture medium (n = 4), or cultured at atmospheric (21%) or low (3%) oxygen concentrations (F) (n = 3). *P<0.05, **P<0.01, ***P<0.001.