Ablation of Treg cell leads to decreased fungal loads and diminished dissemination.
(A) Groups of five C57BL/6Foxp3GFP mice were given anti-CD25 or control IgG antibodies and inoculated with 106P. brasiliensis yeast cells. The frequency of lung-derived CD4+CD25+ cells at weeks 2 and 10 post-infection is indicated. (B) Frequency and number of conventional CD4+ T cells as well as of Treg cells in the lungs at weeks 2 and 10 after infection following administration of control IgG or anti-CD25 antibody. Bars represent mean ± SD of three independent experiments with four to six mice per group (** p < 0.005; *** p < 0.001). (C) Fungal burden in organs of mice infected with Pb18 and treated with anti-CD25 or control IgG, after 2 (left) and 10 (right) weeks of infection. Horizontal bars indicate the mean value in each group (**p < 0.05, ***p < 0.005; n ≥ 15).