A targeted RNAi screen identifies 17 RNAi candidates that decrease the lifespan of the isp-1;ctb-1 mutant.

The mean fold change in lifespan relative to that of wild-type worms treated with empty vector (black bar, value set to 1) was calculated for isp-1;ctb-1 mutant treated with empty vector or with each RNAi clone tested. The mean fold changes (± s.d.) presented are averaged from at least three independent experiments. Only the RNAi clones that decrease the lifespan of the isp-1;ctb-1 mutant by at least 10% are represented (see Table 1). Among them, 13 represent transcription factors not previously known to function in aging and four (indicated with asterisks) are transcription factors already known to affect lifespan in C. elegans. Quantitative data and statistical analyses for the experiments shown here are included in Tables 1 and S1.