Figure_7.tif (1.55 MB)
A pool of ARL2 localizes to the mitochondrial matrix.
figure
posted on 2014-06-09, 03:35 authored by Laura E. Newman, Cheng-jing Zhou, Samatha Mudigonda, Alexa L. Mattheyses, Eleonora Paradies, Carlo Marya Thomas Marobbio, Richard A. KahnHeLa cells were fixed in 4% paraformaldehyde prior to permeabilization in either 0.02% (two upper rows) or 0.1% w/v digitonin (bottom row) for 10 minutes at room temperature. Cells were then processed for imaging using dual labeling for ARL2 (green, left) and either cytochrome c (top row, middle panel) or HSP60 (lower two rows, middle panels), as markers of the IMS and matrix, respectively. Merged images are shown on the far right in each case.