Public Library of Science
Browse
Figure_1.tif (1.4 MB)

(A) Intrinsic transcript cleavage property of RNAP.

Download (0 kB)
figure
posted on 2011-07-08, 02:11 authored by Arnab China, Sonakshi Mishra, Valakunja Nagaraja

Stalled elongation complexes bearing the 20 mer transcript were generated with M. smegmatis (Ms), M. tuberculosis (Mtb) and E. coli (Ec) RNAP respectively. The complexes were incubated for a prolonged time (1–4 hrs) in transcription buffer (pH 7.5), followed by resolving the cleavage products on 20% urea-PAGE. (B) pH-induced transcript-cleavage activity of RNAP. The gels show cleaved RNA generated from the 20 mer ternary complexes formed by Ms, Mtb and Ec RNAP in buffers of pH 6.0 to 10.0.

History

Usage metrics

    PLOS ONE

    Licence

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC