AZT-mediated increments of oxidative stress markers.
(A) Equal amounts of mitochondrial proteins (50 µg/lane) were used to evaluate the levels of mitochondrial iNOS (upper panel) and ATP synthase (lower panel) from both groups. (B) The densities of iNOS bands were normalized to that of ATP synthase, the loading control. (C) The rate of production of hydrogen peroxide and (D) the level of MDA+HAE as a marker of lipid peroxidation was measured as described in the Materials and Methods. *Significantly different from the control group. Data indicate mean±SE, p<0.05. All experiments have been conducted three times.